Local view for "https://dbmi-icode-01.dbmi.pitt.edu/dikb/resource/Evidence/1050"

PredicateValue (sorted: default)
rdfs:label
"evidence_1980"
rdf:type
?:Evidence
?:Item
?:Evidence_enzyme_system
""
?:Evidence_type
?:EV_EX_Met_Enz_ID_Cyp450_Hum_Recom
?:content
"Enzyme system: recombinant human CYP enzymes in baculovirus-infected insect cells NADPH added: yes Quote: cDNA-expressed CYP isoforms were used to identify the isoforms that are able to mediate the N-dealkylation of perphenazine, which is considered a major metabolic pathway for the drug. Using human liver microsomal preparations (HLM), inhibition studies were carried out to establish the relative contributions of the CYP isoforms involved in the N-dealkylation reaction. RESULTS: CYP isoforms 1A2, 3A4, 2C8, 2C9, 2C18, 2C19 and 2D6 were able to mediate the N-dealkylation of perphenazine. Reaction velocities and their relative abundance in HLM suggested that CYP1A2, 3A4, 2C19 and 2D6 were the most important contributors to N-dealkylation. Apparent Km values of CYP1A2 and CYP2D6 were in the range 1-2 microM, and Km values of CYP2C19 and CYP3A4 were 14 microM and 7.9 microM, respectively."
dc:creator
"boycer"
dc:date
"01132010"
rdfs:seeAlso
?:11136295

All properties reside in the graph file:///home/swish/src/ClioPatria/guidelines/dikb.ttl

The resource appears as object in one triple:

{ perphenazine_is_not_substrate_of_cyp2c19, <http://purl.org/swan/1.2/swan-commons#citesAsRefutingEvidence>, evidence_1980 }

Context graph